LS-B2540

antibody from LSBio

Targeting: LEFTY2 EBAF, LEFTA, LEFTYA, TGFB4

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B2540

Provider

LSBio

Proper citation

LifeSpan Cat#LS-B2540, RRID:AB_2135790

Product name

IHC-plus™ TGFB4 / LEFTY2 Antibody (clone 7C5G1H6H10) LS-B2540

Antibody type

Monoclonal

Description

Protein A purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

7C5G1H6H10

Storage

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Mab anti-Human LEFTY antibody (clone 7C5G1H6H10) is shown to detect by western blot partially purified recombinant 6X His tagged human LEFTY. 1.0 mg of protein of protein was loaded per lane. The blot was incubated with a 1:2000 dilution of Mab anti-Human LEFTY.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Mab anti-Human LEFTY antibody (clone 7C5G1H6H10) is shown to detect by western blot partially purified recombinant 6X His tagged human LEFTY. Detection occurs after 1.0 ug of protein is loaded in each lane. The blot was incubated with a 1:2000 dilution of Mab anti-Human LEFTY at room temperature for 30 min followed by detection using IRDye800 labeled Goat-a-Mouse IgG [H&L] (610-132-121) diluted 1:1000. Lane 1 contains an unrelated 6X His tagged protein and shows that the antibody does not recognize the epitope tag. Lane 2 contains partially purified recombinant human LEFTY. The antibody may be used to detect endogenous human LEFTY. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.