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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB14555 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB14555, RRID:AB_10549969
- Product name
- EGLN1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of EGLN1.
- Storage
- Store at 4°C. Do not freeze.
Submitted references Concordant regulation of gene expression by hypoxia and 2-oxoglutarate-dependent dioxygenase inhibition: the role of HIF-1alpha, HIF-2alpha, and other pathways.
Overexpression and nuclear translocation of hypoxia-inducible factor prolyl hydroxylase PHD2 in head and neck squamous cell carcinoma is associated with tumor aggressiveness.
Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung.
Elvidge GP, Glenny L, Appelhoff RJ, Ratcliffe PJ, Ragoussis J, Gleadle JM
The Journal of biological chemistry 2006 Jun 2;281(22):15215-26
The Journal of biological chemistry 2006 Jun 2;281(22):15215-26
Overexpression and nuclear translocation of hypoxia-inducible factor prolyl hydroxylase PHD2 in head and neck squamous cell carcinoma is associated with tumor aggressiveness.
Jokilehto T, Rantanen K, Luukkaa M, Heikkinen P, Grenman R, Minn H, Kronqvist P, Jaakkola PM
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Feb 15;12(4):1080-7
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Feb 15;12(4):1080-7
Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung.
Asikainen TM, Schneider BK, Waleh NS, Clyman RI, Ho WB, Flippin LA, Günzler V, White CW
Proceedings of the National Academy of Sciences of the United States of America 2005 Jul 19;102(29):10212-7
Proceedings of the National Academy of Sciences of the United States of America 2005 Jul 19;102(29):10212-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of human EGLN1, using EGLN1 polyclonal antibody (Cat # PAB14555).Lane 1 : Recombinant FLAG-His-PHD1 (10ng/lane).Lane 2 : PHD2 (10ng/lane) .Lane 3 : PHD3 (10ng/lane).Lane 4 : Whole cell lysate from HeLa cells.Lane 5 : Hela with hypoxia treatment.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Staining of Vascular Endothelium 40X in human lung using EGLN1 polyclonal antibody (Cat # PAB14555).
- Validation comment
- Immunohistochemistry
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric detection of EGLN1. 106 Jurkat cells were fixed, permeabilized, and stained with 3.0 ug/mL EGLN1 polyclonal antibody (Cat # PAB14555) in a 150 uL reaction.
- Validation comment
- Flow Cytometry
