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Learn25287-1-AP
antibody from Invitrogen Antibodies
Targeting: FER
PPP1R74, TYK3
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [6]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 25287-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FER Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: PLHRLTMMI KDKQQVKKSY IGVHQQIEAE MIKVTKTELE KLKCSYRQLI KEMNSAKEKY KEALAKGKET EKAKERYDKA TMKLHMLHNQ YVLALKGAQL HQNQYYDITL PLLLDSLQKM QEEMIKALKG IFDEYSQITS LVTEEIVNVH KEIQMSVEQI DPSTEYNNFI DVHRTTAAKE QEIEFDTSLL EENENLQANE IMWNNLTAES LQVMLKTLAE ELMQTQQMLL NKEEAVLELE KRIEESSETC EKKSDIVLLL SQKQALEELK QSVQQLRCTE AKFSAQKELL EQKVQENDGK EPPPVVNYEE DARSVTSMER KERLSKFESI RHSIAGIIRS PKSALGSSAL S (96-445 aa encoded by BC017060)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.43 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 25287-1-AP (FER Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25287-1-AP ( FER Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 25287-1-AP (FER Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells using 25287-1-AP (FER antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells using 25287-1-AP (FER antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon tissue slide using 25287-1-AP (FER Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon tissue slide using 25287-1-AP (FER Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 25287-1-AP (FER Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 25287-1-AP (FER Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 25287-1-AP ( FER Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 25287-1-AP ( FER Antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 Jurkat cells were stained with 0.5ug FER antibody (25287-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-FER (IP:25287-1-AP, 4ug; Detection:25287-1-AP 1:1000) with MCF-7 cells lysate 2400ug.
