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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 691102 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#691102, RRID:AB_2629828
- Product name
- Purified anti-CAMK IV
- Antibody type
- Monoclonal
- Description
- Calcium/calmodulin-dependent protein kinase IV (CaMK IV) is a multifunctional serine/threonine kinase regulating different cellular processes. CaMK IV resides in both the nucleus and cytosol, but the active phosphorylated form of CaMK IV is predominantly nuclear. Once activated, CAMK IV enhances BCL2 gene expression. CaMK IV also actively regulates gene transcription by phosphorylating HDAC4. Phosphorylation of HDAC4 by CaMK IV promotes HDAC4 nuclear exports in neurons, resulting in major cell death mechanism in cerebral ischemia. CaMK IV–mTOR-dependent autophagy was fundamentally important for IL-6 production in vitro and in vivo.CaMK IV is abnormally increased in T cells from patients with SLE and lupus-prone mice. Genetic or pharmacologic inhibition of CaMK IV resulted in a significant decrease of autoantibody production, mesangial cell proliferation, improved Treg function, improvement of lupus-related pathology, and the survival rates. CaMK IV inhibition has potential as a therapeutic strategy for Th17-driven autoimmune diseases.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- P88C5B8
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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- BioLegend (provider)
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- Experimental details
- Total cell or tissue lysates (15 µg protein) from EL-4, Jurkat, HepG2, and NIH3T3 were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-CAMK IV antibody (clone P88C5B8) antibody (upper) or loading control GAPDH (clone poly6314) antibody (lower). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated
