Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00000814-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000814-M01, RRID:AB_537378
- Product name
- Mouse Monoclonal CaMKIV Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CAMK4 - calcium/calmodulin-dependent protein kinase IV
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Acid activation of Trpv1 leads to an up-regulation of calcitonin gene-related peptide expression in dorsal root ganglion neurons via the CaMK-CREB cascade: a potential mechanism of inflammatory pain.
Nakanishi M, Hata K, Nagayama T, Sakurai T, Nishisho T, Wakabayashi H, Hiraga T, Ebisu S, Yoneda T
Molecular biology of the cell 2010 Aug 1;21(15):2568-77
Molecular biology of the cell 2010 Aug 1;21(15):2568-77
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CaMKIV Antibody (1A3) [H00000814-M01] - Analysis of CAMK4 over-expressed 293 cell line, cotransfected with CAMK4 Validated Chimera RNAi ( Cat # H00000814-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMK4 monoclonal antibody (M01) clone 1A3 (Cat # H00000814-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CaMKIV Antibody (1A3) [H00000814-M01] - Analysis of CAMK4 expression in transfected 293T cell line by CAMK4 monoclonal antibody (M01), clone 1A3.Lane 1: CAMK4 transfected lysate(52.14 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CaMKIV Antibody (1A3) [H00000814-M01] - CAMK4 monoclonal antibody (M01), clone 1A3 Analysis of CAMK4 expression in Jurkat.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: CaMKIV Antibody (1A3) [H00000814-M01] - Analysis of monoclonal antibody to CAMK4 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CaMKIV Antibody (1A3) [H00000814-M01] - Analysis of monoclonal antibody to CAMK4 on formalin-fixed paraffin-embedded human tonsil tissue. Antibody concentration 5 ug/ml.