AP09079SU-N

antibody from Acris Antibodies GmbH

Targeting: CAMK4 CaMK-GR, CaMKIV

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP09079SU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09079SU-N, RRID:AB_2035240

Product name

anti CAMK4 (305-323)

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to amino acids 305-323 of Human CaM Kinase IV protein

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Zebrafish

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

70 mg/ml by Refractometry

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using Anti-CaM Kinase IV antibody shows detection of a band ~52 kDa corresponding to CaM Kinase IV (arrowhead) in various preparations. Staining of rat brain lysate is shown in lane 1. Jurkat cell lysate staining is shown in lane 2. Specific reactivity is blocked in both lysates when antibody is preincubated with immunizing peptide (lanes 3 and 4 respectively). Approximately 35 µg of each lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. CaM Kinase IV was similarly detected on lysates from mouse brain (not shown). After blocking the membrane was probed with the primary antibody diluted to 1:1,000 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry. Anti-CAMK4 antibody was diluted 1:500 to detect CAMK4 in human brain cortex tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.