H00002049-M02

antibody from Abnova Corporation

Targeting: EPHB3 ETK2, Hek2, Tyro6

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002049-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002049-M02, RRID:AB_530035

Product name

EPHB3 monoclonal antibody (M02), clone 1E12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant EPHB3.

Antigen sequence

AASLKVIASAQSGMSQPLLDRTVPDYTTFTTVGDW
LDAIKMGRYKESFVSAGFASFDLVAQMTAEDLLRI
GVTLAGHQKKILSSIQDMRLQMNQTLPVQ


Isotype

IgG

Antibody clone number

1E12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

EPHB3 monoclonal antibody (M02), clone 1E12 Western Blot analysis of EPHB3 expression in HeLa ( Cat # L013V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged EPHB3 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to EPHB3 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol