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antibody from Invitrogen Antibodies
Targeting: LRRK2
DKFZp434H2111, FLJ45829, PARK8, RIPK7, ROCO2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Flow cytometry [3]
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- Product number
- PA1-16770 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LRRK2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The target sequence has 87% sequence homology with rat.
- Reactivity
- Human, Mouse, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Knockdown transgenic Lrrk Drosophila resists paraquat-induced locomotor impairment and neurodegeneration: A therapeutic strategy for Parkinson's disease.
Quintero-Espinosa D, Jimenez-Del-Rio M, Velez-Pardo C
Brain research 2017 Feb 15;1657:253-261
Brain research 2017 Feb 15;1657:253-261
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot detection of LRRK2 in HeLa whole cell lysate (RIPA) using Product # PA1-16770. 1:5,000 dilution, 1 minute ECL Western Blot detection.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of LRRK2 followed by an autophosphorylation assay using Product # PA1-16770. Lane 1 Rabbit IgG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse CAD cells transfected with Human wild-type LRRK-2 (1:2,000)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of LRRK2 in human brain, Putamen, Neurons and Glia using a 60X magnification. Samples were incubated in LRRK2 polyclonal antibody (Product # PA1-16770).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of LRRK2 in Neuro2a cells (blue) and a matched isotype control (orange). Samples were incubated in LRRK2 polyclonal antibody (Product # PA1-16770) using a dilution of 2.5 µg/mL for 30 minutes at room temperature followed by a Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight™ 550 (Product # SA5-10033). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of LRRK2 in U2OS cells (blue) and a matched isotype control (orange). Samples were incubated in LRRK2 polyclonal antibody (Product # PA1-16770) using a dilution of 2.5 µg/mL for 30 minutes at room temperature followed by a Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight™ 550 (Product # SA5-10033). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of LRRK2 in U2OS cells. Samples were incubated in LRRK2 polyclonal antibody (Product # PA1-16770) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to Alexa Fluor 647.
