HPA009143

antibody from Atlas Antibodies

Targeting: COL12A1 COL12A1L

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA009143

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA009143, RRID:AB_1847095

Product name

Anti-COL12A1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

VTTPPNQRRRTLENLIPDTKYEVSVIPEYFSGPGT
PLTGNAATEEVRGNPRDLRVSDPTTSTMKLSWSGA
PGKVKQYLVTYTPVAGGETQEVTVRGDTTNTVLQG
LKEGTQYALSVTALYASGAG


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines U2OS and A-431 using Anti-COL12A1 antibody. Corresponding COL12A1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line SiHa.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows weak cytoplasmic positivity in Leydig cells and cells in seminiferous ducts.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human endometrium shows strong positivity in basement membranes.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows very weak cytoplasmic positivity in hepatocytes.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows strong membranous positivity in peritubular myoid cells.

Sample type

HUMAN