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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00009659-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00009659-M01, RRID:AB_425815
- Product name
- PDE4DIP monoclonal antibody (M01), clone 2B5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant PDE4DIP.
- Antigen sequence
MFLCQGFRKYLPEHLNDLKKENFSLKLRIYFLEER
MQQKYEASREDIYRRNTELKVEVESLKRELQDKKQ
HLDKTWADVENLNSQNEAELRRQFEERQQETEHVY
ELLENKMQLLQEESRLAKNEAARMAALVEAEKECN
LELSEKLKGVTKNWEDVPGDQVKPDQYTEALAQRD
KI- Isotype
- IgG
- Antibody clone number
- 2B5
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PDE4DIP monoclonal antibody (M01), clone 2B5 Western Blot analysis of PDE4DIP expression in JAR ( Cat # L003V1 ).
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PDE4DIP is 0.03 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to PDE4DIP on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
