Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF235 - Provider product page
- Provider
- R&D Systems
- Product name
- Human FGF-4 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human FGF-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) FGF-6 is observed and less than 2% cross-reactivity with bovine FGF acidic, bovine FGF basic, rhFGF acidic, rhFGF basic, rh beta-ECGF, rhFGF-5, rhFGF-7, rhFGF-8b, recombinant mouse (rm) FGF-8b, rmFGF-8c, and rhFGF-10 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P08620
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- FGF-4 in Human Breast Cancer Tissue. FGF-4 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human FGF-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF235) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by FGF-4 and Neutralization by Human FGF-4 Antibody. Recombinant Human FGF-4 (Catalog # 235-F4) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF235). The ND50 is typically 0.3-0.9 µg/mL.