AF3940

antibody from Novus Biologicals

Targeting: TSKU E2IG4, LRRC54, TSK

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3940

Provider

Novus Biologicals

Product name

Goat Polyclonal Tsukushi/TSK Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human Tsukushi/TSK in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse TSK is observed.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Tsukushi/TSK in Human Colon Cancer Tissue. Tsukushi/TSK was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human Tsukushi/TSK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3940) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to smooth muscle. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.