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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- ABIN1449617 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-UV Radiation Resistance Associated Gene (UVRAG) (C-Term), (AA 670-699) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 670~699 amino acids from the C-term of Human UVRAG
- Description
- Saturated Ammonium Sulfate precipitation followed by dialysis against PBS
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Epitope
- C-Term,AA 670-699
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Identification of Barkor as a mammalian autophagy-specific factor for Beclin 1 and class III phosphatidylinositol 3-kinase.
Sun Q, Fan W, Chen K, Ding X, Chen S, Zhong Q
Proceedings of the National Academy of Sciences of the United States of America 2008 Dec 9;105(49):19211-6
Proceedings of the National Academy of Sciences of the United States of America 2008 Dec 9;105(49):19211-6
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunofluorescence staining of Autophagy UVRAG Antibody Cat.-No AP32185PU-N on Methanol-fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki, Finland
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunoprecipitation and Western blot analysis of anti-UVRAG Antibody Cat.-No AP32185PU-N in 293T cells. UVRAG is immunoprecipitated (Lane 2) and detected in 293T cell transiently transfected with Mouse UVRAG (Lane 1). Detection of endogenous UVRAG is shown in 293T cells (Lane 3) but is reduced by UVRAG siRNA transfection (Lane 4). Data courtesy of Dr. Hong-Gang Wang, Moffitt Cancer Center, Tampa, FL.Western Blot: Anti-UVRAG Antibody Cat.-No AP32185PU-N. Immunoprecipitation: Anti-UVRAG Antibody Cat.-No AP32185PU-N.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with UVRAG Antibody Cat.-No AP32185PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
