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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB100-899 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-899, RRID:AB_2091630
- Product name
- Goat Polyclonal Dkk-3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. NP_037385.2
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
C-AAAALLGGEEI- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Functional and structural diversity of the human Dickkopf gene family.
Functional and structural diversity of the human Dickkopf gene family.
Krupnik VE, Sharp JD, Jiang C, Robison K, Chickering TW, Amaravadi L, Brown DE, Guyot D, Mays G, Leiby K, Chang B, Duong T, Goodearl AD, Gearing DP, Sokol SY, McCarthy SA
Gene 1999 Oct 1;238(2):301-13
Gene 1999 Oct 1;238(2):301-13
Functional and structural diversity of the human Dickkopf gene family.
Krupnik VE, Sharp JD, Jiang C, Robison K, Chickering TW, Amaravadi L, Brown DE, Guyot D, Mays G, Leiby K, Chang B, Duong T, Goodearl AD, Gearing DP, Sokol SY, McCarthy SA
Gene 1999 Oct 1;238(2):301-13
Gene 1999 Oct 1;238(2):301-13
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Dkk-3 Antibody [NB100-899] - (2ug/ml) of HEK293 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Dkk-3 Antibody [NB100-899] - Staining of Human Cerebellum lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 2 ug/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Dkk-3 Antibody [NB100-899] - (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
