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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [10]
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- Western blot [1]
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- Product number
- AF1118 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Dkk-3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Dkk-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant mouse Dkk-3 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9UBP4- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Fatty Acid Binding Protein 4 (FABP4) Overexpression in Intratumoral Hepatic Stellate Cells within Hepatocellular Carcinoma with Metabolic Risk Factors.
Loss of Dickkopf 3 Promotes the Tumorigenesis of Basal Breast Cancer.
Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
Epigenetic silencing of the WNT antagonist Dickkopf 3 disrupts normal Wnt/β-catenin signalling and apoptosis regulation in breast cancer cells.
The clinicopathological significance of REIC expression in colorectal carcinomas.
The fibroblast-derived paracrine factor neuregulin-1 has a novel role in regulating the constitutive color and melanocyte function in human skin.
Regulation of human skin pigmentation in situ by repetitive UV exposure: molecular characterization of responses to UVA and/or UVB.
Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development.
Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development.
The Dickkopf-homolog 3 is expressed in tumor endothelial cells and supports capillary formation.
Chiyonobu N, Shimada S, Akiyama Y, Mogushi K, Itoh M, Akahoshi K, Matsumura S, Ogawa K, Ono H, Mitsunori Y, Ban D, Kudo A, Arii S, Suganami T, Yamaoka S, Ogawa Y, Tanabe M, Tanaka S
The American journal of pathology 2018 May;188(5):1213-1224
The American journal of pathology 2018 May;188(5):1213-1224
Loss of Dickkopf 3 Promotes the Tumorigenesis of Basal Breast Cancer.
Lorsy E, Topuz AS, Geisler C, Stahl S, Garczyk S, von Stillfried S, Hoss M, Gluz O, Hartmann A, Knüchel R, Dahl E
PloS one 2016;11(7):e0160077
PloS one 2016;11(7):e0160077
Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
Riordan DP, Varma S, West RB, Brown PO
PloS one 2015;10(7):e0128975
PloS one 2015;10(7):e0128975
Epigenetic silencing of the WNT antagonist Dickkopf 3 disrupts normal Wnt/β-catenin signalling and apoptosis regulation in breast cancer cells.
Xiang T, Li L, Yin X, Zhong L, Peng W, Qiu Z, Ren G, Tao Q
Journal of cellular and molecular medicine 2013 Oct;17(10):1236-46
Journal of cellular and molecular medicine 2013 Oct;17(10):1236-46
The clinicopathological significance of REIC expression in colorectal carcinomas.
Wang W, Zhu W, Xu XY, Nie XC, Yang X, Xing YN, Yu M, Liu YP, Takano Y, Zheng HC
Histology and histopathology 2012 Jun;27(6):735-43
Histology and histopathology 2012 Jun;27(6):735-43
The fibroblast-derived paracrine factor neuregulin-1 has a novel role in regulating the constitutive color and melanocyte function in human skin.
Choi W, Wolber R, Gerwat W, Mann T, Batzer J, Smuda C, Liu H, Kolbe L, Hearing VJ
Journal of cell science 2010 Sep 15;123(Pt 18):3102-11
Journal of cell science 2010 Sep 15;123(Pt 18):3102-11
Regulation of human skin pigmentation in situ by repetitive UV exposure: molecular characterization of responses to UVA and/or UVB.
Choi W, Miyamura Y, Wolber R, Smuda C, Reinhold W, Liu H, Kolbe L, Hearing VJ
The Journal of investigative dermatology 2010 Jun;130(6):1685-96
The Journal of investigative dermatology 2010 Jun;130(6):1685-96
Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development.
Zhang K, Watanabe M, Kashiwakura Y, Li SA, Edamura K, Huang P, Yamaguchi K, Nasu Y, Kobayashi Y, Sakaguchi M, Ochiai K, Yamada H, Takei K, Ueki H, Huh NH, Li M, Kaku H, Na Y, Kumon H
International journal of oncology 2010 Dec;37(6):1495-501
International journal of oncology 2010 Dec;37(6):1495-501
Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development.
Zhang K, Watanabe M, Kashiwakura Y, Li SA, Edamura K, Huang P, Yamaguchi K, Nasu Y, Kobayashi Y, Sakaguchi M, Ochiai K, Yamada H, Takei K, Ueki H, Huh NH, Li M, Kaku H, Na Y, Kumon H
International journal of oncology 2010 Dec;37(6):1495-501
International journal of oncology 2010 Dec;37(6):1495-501
The Dickkopf-homolog 3 is expressed in tumor endothelial cells and supports capillary formation.
Untergasser G, Steurer M, Zimmermann M, Hermann M, Kern J, Amberger A, Gastl G, Gunsilius E
International journal of cancer 2008 Apr 1;122(7):1539-47
International journal of cancer 2008 Apr 1;122(7):1539-47
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Dkk-3 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Dkk-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1118) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Dkk-3 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
