Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ACP-005-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PMCA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 25 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-PMCA1 Antibody (#ACP-005), (1:200).3,4. Anti-PMCA1 Antibody , preincubated with PMCA1 Blocking Peptide (#BLP-CP005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-PMCA1 Antibody (#ACP-005), (1:200).3,4. Anti-PMCA1 Antibody , preincubated with PMCA1 Blocking Peptide (#BLP-CP005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of PMCA1 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-PMCA1 Antibody (#ACP-005), (1:300), followed by goat Anti-rabbit-AlexaFluor-488 Antibody . PMCA1 staining (green) appears in the stratum radiatum (SR) and oriens (OR) layers but not in the pyramidal (Pyr) layer of hippocampal CA1 region (arrows). Nuclei are labeled with DAPI (blue).