H00009114-M01

antibody from Abnova Corporation

Targeting: ATP6V0D1 ATP6D, ATP6DV, P39, VATX, Vma6, VPATPD

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00009114-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00009114-M01, RRID:AB_509245

Product name

ATP6V0D1 monoclonal antibody (M01), clone 2G12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant ATP6V0D1.

Antigen sequence

AKLFPHCGRLYPEGLAQLARADDYEQVKNVADYYP
EYKLLFEGAGSNPGDKTLEDRFFEHEVKLNKLAFL
N


Isotype

IgG

Antibody clone number

2G12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

ATP6V0D1 monoclonal antibody (M01), clone 2G12 Western Blot analysis of ATP6V0D1 expression in HeLa ( Cat # L013V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of ATP6V0D1 expression in transfected 293T cell line by ATP6V0D1 monoclonal antibody (M01), clone 2G12.Lane 1: ATP6V0D1 transfected lysate(40.3 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ATP6V0D1 is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to ATP6V0D1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 0.5 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol