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Learn18274-1-AP
antibody from Invitrogen Antibodies
Targeting: ATP6V0D1
ATP6D, ATP6DV, P39, VATX, Vma6, VPATPD
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 18274-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP6V0D1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSFFPELYF NVDNGYLEGL VRGLKAGVLS QADYLNLVQC ETLEDLKLHL QSTDYGNFLA NEASPLTVSV IDDRLKEKMV VEFRHMRNHA YEPLASFLDF ITYSYMIDNV ILLITGTLHQ RSIAELVPKC HPLGSFEQME AVNIAQTPAE LYNAILVDTP LAAFFQDCIS EQDLDEMNIE IIRNTLYKAY LESFYKFCTL LGGTTADAMC PILEFEADRR AFIITINSFG TELSKEDRAK LFPHCGRLYP EGLAQLARAD DYEQVKNVAD YYPEYKLLFE GAGSNPGDKT LEDRFFEHEV KLNKLAFLNQ FHFGVFYAFV KLKEQECRNI VWIAECIAQR HRAKIDNYIP IF (1-351 aa encoded by BC008861)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.3 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human placenta tissue were subjected to SDS PAGE followed by western blot with 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug ATP6V0D1 antibody (18274-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ATP6V0D1 (IP:18274-1-AP, 4ug; Detection:18274-1-AP 1:500) with mouse testis tissue lysate 4800ug.
