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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- NBP1-47612 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-47612, RRID:AB_10010569
- Product name
- Mouse Monoclonal GKAP/DLGAP1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects approx. 120kDa (SAPAP2), approx. 110kDa (SAPAP1/3/4). It does cross-react with other SAPAPs .
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Zinc deficiency dysregulates the synaptic ProSAP/Shank scaffold and might contribute to autism spectrum disorders.
Grabrucker S, Jannetti L, Eckert M, Gaub S, Chhabra R, Pfaender S, Mangus K, Reddy PP, Rankovic V, Schmeisser MJ, Kreutz MR, Ehret G, Boeckers TM, Grabrucker AM
Brain : a journal of neurology 2014 Jan;137(Pt 1):137-52
Brain : a journal of neurology 2014 Jan;137(Pt 1):137-52
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: GKAP/DLGAP1 Antibody (S127-31) [NBP1-47612] - analysis of Rat brain membrane lysate showing detection of SAPAP protein using Mouse Anti-SAPAP Monoclonal Antibody, Clone S127-31 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SAPAP Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
