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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- H00056907-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00056907-M01, RRID:AB_2255207
- Product name
- Mouse Monoclonal SPIRE1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SPIRE1 - spire homolog 1 (Drosophila)
- Reactivity
- Human, Hamster
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Atypical function of a centrosomal module in WNT signalling drives contextual cancer cell motility.
Annexin A2-dependent polymerization of actin mediates endosome biogenesis.
Actin S-nitrosylation inhibits neutrophil beta2 integrin function.
Luo Y, Barrios-Rodiles M, Gupta GD, Zhang YY, Ogunjimi AA, Bashkurov M, Tkach JM, Underhill AQ, Zhang L, Bourmoum M, Wrana JL, Pelletier L
Nature communications 2019 May 29;10(1):2356
Nature communications 2019 May 29;10(1):2356
Annexin A2-dependent polymerization of actin mediates endosome biogenesis.
Morel E, Parton RG, Gruenberg J
Developmental cell 2009 Mar;16(3):445-57
Developmental cell 2009 Mar;16(3):445-57
Actin S-nitrosylation inhibits neutrophil beta2 integrin function.
Thom SR, Bhopale VM, Mancini DJ, Milovanova TN
The Journal of biological chemistry 2008 Apr 18;283(16):10822-34
The Journal of biological chemistry 2008 Apr 18;283(16):10822-34
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SPIRE1 Antibody (4C5) [H00056907-M01] - Analysis of SPIRE1 expression in transfected 293T cell line by SPIRE1 monoclonal antibody (M01), clone 4C5.Lane 1: SPIRE1 transfected lysate(15 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: SPIRE1 Antibody (4C5) [H00056907-M01] - Detection limit for recombinant GST tagged SPIRE1 is approximately 0.03ng/ml as a capture antibody.
