H00026260-M01

antibody from Abnova Corporation

Targeting: FBXO25 FBX25

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00026260-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00026260-M01, RRID:AB_425931

Product name

FBXO25 monoclonal antibody (M01), clone 2F1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant FBXO25.

Antigen sequence

MPFLGQDWRSPGWSWIKTEDGWKRCESCSQKLERE
NNHCNISHSIILNSEDGEIFNNEEHEYASKKRKKD
HFRNDTNTQSFYREKWIYVHKESTKERHGYCTLGE
AFNRLDFSSAIQDIRRFNYVVKLLQLIAKSQLTSL
SGVAQKNYFNILDKIVQKVLDDHHNPRLIKDLLQD
LSSTLCILIRGVGKSVLVGNINIWICRLETILAWQ
QQLQDLQMTKQVNNGLTLSDLPLHMLNNILYRFSD
GWDIITLGQVTPTLYMLSEDRQLWKKLCQYHFAEK
QFCRHLILSEKGHIEWKLMYFALQKHYPAKEQYGD
TLHFCRHCSILFWKDYHLALLFKDSGHPCTAADPD
SCFTPVSPQHFIDLFKF


Isotype

IgG

Antibody clone number

2F1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

FBXO25 monoclonal antibody (M01), clone 2F1 Western Blot analysis of FBXO25 expression in COLO 320 HSR ( Cat # L020V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of FBXO25 expression in transfected 293T cell line by FBXO25 monoclonal antibody (M01), clone 2F1.Lane 1: FBXO25 transfected lysate(43 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged FBXO25 is approximately 3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol