GTX22864

antibody from GeneTex

Targeting: CACNA2D1 CACNA2, CACNL2A, LINC01112, lncRNA-N3, MHS3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX22864

Provider

GeneTex

Proper citation

GeneTex Cat#GTX22864, RRID:AB_384883

Product name

CACNA2D1 antibody [20A]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Guinea Pig, Porcine, Rabbit

Host

Mouse

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of Cav1.1 in 25 ug of U87-MG and mouse brain lysates. Proteins were transferred to a PVDF membrane and blocked at 4¢XC overnight. The membrane was probed with Cav1.1 antibody [1A] at a dilution of 1:1000 overnight at 4¢XC, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of Cav1.1 in HeLa cells. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Cav1.1 antibody [1A] at a dilution of 1:100 overnight at 4¢XC, washed with PBS and incubated with a proper secondary antibody. Cav1.1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Flow cytometry analysis of Cav1.1 in C6 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with Cav1.1 antibody [1A] at a dilution of 1:100 for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a proper secondary antibody and re-suspended in PBS for FACS analysis.