18030-1-AP

antibody from Invitrogen Antibodies

Targeting: TNKS ARTD5, PARP-5a, PARP5A, pART5, TIN1, TINF1, TNKS1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

18030-1-AP

Provider

Invitrogen Antibodies

Product name

TNKS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAASRRSQH HHHHHQQQLQ PAPGASAPPP PPPPPLSPGL APGTTPASPT ASGLAPFASP RHGLALPEGD GSRDPPDRPR SPDQVDGTSC CSTTSTICTV AAAPVVPAVS TSSAAGVAPN PAGSGSNNSP SSSSSPTSSS SSSPSSPGSS LAESPEAAGV SSTAPLGPGA AGPGTGVPAV SGALRELLEA CRNGDVSRVK RLVDAANVNA KDMAGRKSSP LHFAAGFGRK DVVEHLLQMG ANVHARDDGG LIPLHNACSF GHAEVVSLLL CQGADPNARD NWNYTPLHEA AIKGKIDVCI VLLQHGADPN IRNTDGKSAL DLADPSAKAV (1-329 aa encoded by BC098394)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 18030-1-AP (TNKS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF-7 cells were subjected to SDS PAGE followed by western blot with 18030-1-AP (TNKS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using TNKS antibody 18030-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 18030-1-AP (TNKS antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 18030-1-AP (TNKS antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-TNKS (IP:18030-1-AP, 4ug; Detection:18030-1-AP 1:500) with Jurkat cells lysate 3200ug.