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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-59805 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IMPACT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ENKKIASATH NIYAYRIYCE DKQTFLQDCE DDGETAAGGR LLHLMEILNV KNVMVVV
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IMPACT in control (vector only transfected HEK293T lysate) and IMPACT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a IMPACT Polyclonal Antibody (Product # PA5-59805).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of IMPACT in human cell line U-251 MG shows positivity in nucleus but excluded from the nucleoli. Samples were probed using an IMPACT Polyclonal Antibody (Product # PA5-59805).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of IMPACT in human testis using an IMPACT Polyclonal Antibody (Product # PA5-59805) shows strong nuclear and cytoplasmic positivity in cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of IMPACT in human prostate using IMPACT Polyclonal Antibody (Product # PA5-59805) shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of IMPACT in human testis using IMPACT Polyclonal Antibody (Product # PA5-59805) shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
