H00007415-M03

antibody from Abnova Corporation

Targeting: VCP CDC48, IBMPFD, p97, TERA

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007415-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007415-M03, RRID:AB_1205165

Product name

VCP monoclonal antibody (M03), clone 4A8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant VCP.

Antigen sequence

MVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTL
IARAVANETGAFFFLINGPEIMSKLAGESESNLRK
AFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERR
IVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALR
RFGRFDREVDIGIPDATGRLEILQIHTKNMKLADD
VDLEQVANETHGHVGADLAALCSEAALQAIRKKMD
LIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSA
LRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVE
HPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIAN
ECQANFISIKGPELLTMWFGESEANVREIFDKARQ
AAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQ
ILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGR
LDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLE
FLAKMTNGFSGADLTEICQRACKLAIRESIESEIR
RERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFA
RRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQ
GGAGPSQGSGGGTGGSVYTEDNDDDLYG


Isotype

IgG

Antibody clone number

4A8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

VCP monoclonal antibody (M03), clone 4A8. Western Blot analysis of VCP expression in Raw 264.7.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

VCP monoclonal antibody (M03), clone 4A8. Western Blot analysis of VCP expression in PC-12.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged VCP is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to VCP on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol