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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- 636802 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#636802, RRID:AB_2214641
- Product name
- Purified anti-p97/VCP
- Antibody type
- Monoclonal
- Antigen
- Recombinant human valosin containing protein
- Description
- p97/VCP (valosin containing protein) belongs to the AAA ATPase family, predicted molecular weight 97 kD. VCP is associated with a variety of cellular activities, including cell cycle control, membrane fusion after mitosis, regulates spindle disassembly at the end of mitosis, nuclear trafficking and the ubiquitin-proteasome degradation pathway. In many instances, specific adaptor proteins with which VCP forms multiprotein complexes direct its role in a given process. This protein is required for the fusion of ER and Golgi membranes. Clone 4G9 has been shown to be useful for western blotting, immunoprecipitation, and immunofluorescence staining of human p97/VCP protein.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Upon receipt, store undiluted at 4°C."
Submitted references Regulation of subtilase cytotoxin-induced cell death by an RNA-dependent protein kinase-like endoplasmic reticulum kinase-dependent proteasome pathway in HeLa cells.
Altered intracellular localization and valosin-containing protein (p97 VCP) interaction underlie ATP7A-related distal motor neuropathy.
Selenoprotein K binds multiprotein complexes and is involved in the regulation of endoplasmic reticulum homeostasis.
Yahiro K, Tsutsuki H, Ogura K, Nagasawa S, Moss J, Noda M
Infection and immunity 2012 May;80(5):1803-14
Infection and immunity 2012 May;80(5):1803-14
Altered intracellular localization and valosin-containing protein (p97 VCP) interaction underlie ATP7A-related distal motor neuropathy.
Yi L, Donsante A, Kennerson ML, Mercer JF, Garbern JY, Kaler SG
Human molecular genetics 2012 Apr 15;21(8):1794-807
Human molecular genetics 2012 Apr 15;21(8):1794-807
Selenoprotein K binds multiprotein complexes and is involved in the regulation of endoplasmic reticulum homeostasis.
Shchedrina VA, Everley RA, Zhang Y, Gygi SP, Hatfield DL, Gladyshev VN
The Journal of biological chemistry 2011 Dec 16;286(50):42937-48
The Journal of biological chemistry 2011 Dec 16;286(50):42937-48
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Overnight nocodazole treated Hela cells stained with purified mouse monoclonal antibody against p97/VCP (clone 4G9), followed by Cy3 conjugated goat anti-mouse IgG and DAPI.
- Conjugate
- Unconjugated
