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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-21516 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FGFR5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, HepG2 membrane extract, FGFRL1-transfected 293T.
- Concentration
- 2.42 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FGFR-5 using A) 30 µg Jurkat whole cell lysate (B) 30 µg Raji whole cell lysate (C) 30 µg K562 whole cell lysate and D) 30 µg NCI-H929 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a FGFR-5 polyclonal antibody (Product # PA5-21516) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FGFR-5 using A) 30 µg 293T whole cell lysate (B) 30 µg A431 whole cell lysate (C) 30 µg HeLa whole cell lysate and D) 30 µg HepG2 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a FGFR-5 polyclonal antibody (Product # PA5-21516) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of FGFR5 was performed by separating 30 µg of HepG2 whole cell and membrane extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a FGFR5 Polyclonal Antibody (Product # PA5-21516) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of FGFR5 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a FGFR5 Polyclonal Antibody (Product # PA5-21516) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of FGFR-5 showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a FGFR-5 polyclonal antibody (Product # PA5-21516) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Oral CA Cal27 xenograft, using FGFRL1 (Product # PA5-21516) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
