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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-31461 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-31461, RRID:AB_2262721
- Product name
- Rabbit Polyclonal FGFR5/FGFRL1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FGF R5/FGFRL1 Antibody [NBP1-31461] - A. Jurkat whole cell lysate/extract. B. 30 Raji whole cell lysate/extract. C. K562 whole cell lysate/extract. D. NCI-H929 whole cell lysate/extract. 10 % SDS-PAGE FGFR-5 antibody dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FGF R5/FGFRL1 Antibody [NBP1-31461] - A. 293T whole cell lysate/extract. B. A431 whole cell lysate/extract. C. HeLa whole cell lysate/extract. D. HepG2 whole cell lysate/extract. 10 % SDS-PAGE FGFR-5 antibody dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FGFR5/FGFRL1 Antibody [NBP1-31461] - HepG2 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FGFR5 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FGFR5/FGFRL1 Antibody [NBP1-31461] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FGFR5 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: FGF R5/FGFRL1 Antibody [NBP1-31461] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FGFR-5 protein stained by FGFR-5 antibody diluted at 1:500.Blue: Hoechst 33342 staining. Scale bar = 10 um.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FGF R5/FGFRL1 Antibody [NBP1-31461] - Paraffin-embedded Oral CA Cal27 xenograft, using antibody at 1:100 dilution.
