LS-C820431

antibody from LSBio

Targeting: SDHA FP, SDH2, SDHF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C820431

Provider

LSBio

Product name

SDHA Antibody (Preservative Free) LS-C820431

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-SDHA rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: HeLa Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 72 kDa. Observed band size: 72 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-SDHA rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: HeLa Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 72 kDa. Observed band size: 72 kDa.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

SDHA was immunoprecipitated using: Lane A: 0.5 mg MCF7 Whole Cell Lysate. Lane B: 0.5 mg HeLa Whole Cell Lysate. Lane C:0.5 mg Jurkat Whole Cell Lysate. 2 uL anti-SDHA rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-SDHA rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 72 kDa. Observed band size: 72 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

SDHA was immunoprecipitated using: Lane A: 0.5 mg MCF7 Whole Cell Lysate. Lane B: 0.5 mg HeLa Whole Cell Lysate. Lane C:0.5 mg Jurkat Whole Cell Lysate. 2 uL anti-SDHA rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-SDHA rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 72 kDa. Observed band size: 72 kDa.