Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-27482 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SDHA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, Molt-4, mouse brain.
- Concentration
- 1.12 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of SDHA using 30 µg of MOLT4 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a SDHA polyclonal antibody (Product # PA5-27482) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western Blot analysis of SDHA was performed by separating 50 µg of Rat tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a SDHA Polyclonal Antibody (Product # PA5-27482) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SDHA Polyclonal Antibody (Product # PA5-27482). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 7.5% SDS PAGE. SDHA Polyclonal Antibody (Product # PA5-27482) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SDHA Polyclonal Antibody detects SDHA protein by Western blot analysis. Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA Polyclonal Antibody (Product # PA5-27482) diluted by 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SDHA showing staining in the mitochondria of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a SDHA polyclonal antibody (Product # PA5-27482) diluted at 1:500. Blue: Hoechst 33343 staining.
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- SDHA Polyclonal Antibody detects SDHA protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SDHA protein stained by SDHA Polyclonal Antibody (Product # PA5-27482) diluted at 1:500. Blue: Hoechst 33343 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SDHA Polyclonal Antibody detects SDHA protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. SDHA Polyclonal Antibody (Product # PA5-27482) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.