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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB110-61586 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-61586, RRID:AB_964704
- Product name
- Mouse Monoclonal NHE3/SLC9A3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This antibody is specific for NHE3. It does not show any cross-reactivity with any other NHE isoforms.
- Reactivity
- Human, Rat, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Role of the EGF receptor in PPARγ-mediated sodium and water transport in human proximal tubule cells.
Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites.
NHE3 and NHERF are targeted to the basolateral membrane in proximal tubules of colchicine-treated rats.
Na+/H+-exchange activity and immunolocalization of NHE3 in rat epididymis.
Chronic effect of parathyroid hormone on NHE3 expression in rat renal proximal tubules.
In vivo PTH provokes apical NHE3 and NaPi2 redistribution and Na-K-ATPase inhibition.
Specific association of megalin and the Na+/H+ exchanger isoform NHE3 in the proximal tubule.
Membrane topology of NHE3. Epitopes within the carboxyl-terminal hydrophilic domain are exoplasmic.
Monoclonal antibodies for high-resolution localization of NHE3 in adult and neonatal rat kidney.
Saad S, Zhang J, Yong R, Yaghobian D, Wong MG, Kelly DJ, Chen XM, Pollock CA
Diabetologia 2013 May;56(5):1174-82
Diabetologia 2013 May;56(5):1174-82
Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites.
Kocinsky HS, Girardi AC, Biemesderfer D, Nguyen T, Mentone S, Orlowski J, Aronson PS
American journal of physiology. Renal physiology 2005 Aug;289(2):F249-58
American journal of physiology. Renal physiology 2005 Aug;289(2):F249-58
NHE3 and NHERF are targeted to the basolateral membrane in proximal tubules of colchicine-treated rats.
Sabolić I, Herak-Kramberger CM, Ljubojević M, Biemesderfer D, Brown D
Kidney international 2002 Apr;61(4):1351-64
Kidney international 2002 Apr;61(4):1351-64
Na+/H+-exchange activity and immunolocalization of NHE3 in rat epididymis.
Bagnis C, Marsolais M, Biemesderfer D, Laprade R, Breton S
American journal of physiology. Renal physiology 2001 Mar;280(3):F426-36
American journal of physiology. Renal physiology 2001 Mar;280(3):F426-36
Chronic effect of parathyroid hormone on NHE3 expression in rat renal proximal tubules.
Girardi AC, Titan SM, Malnic G, Rebouças NA
Kidney international 2000 Oct;58(4):1623-31
Kidney international 2000 Oct;58(4):1623-31
In vivo PTH provokes apical NHE3 and NaPi2 redistribution and Na-K-ATPase inhibition.
Zhang Y, Norian JM, Magyar CE, Holstein-Rathlou NH, Mircheff AK, McDonough AA
The American journal of physiology 1999 May;276(5):F711-9
The American journal of physiology 1999 May;276(5):F711-9
Specific association of megalin and the Na+/H+ exchanger isoform NHE3 in the proximal tubule.
Biemesderfer D, Nagy T, DeGray B, Aronson PS
The Journal of biological chemistry 1999 Jun 18;274(25):17518-24
The Journal of biological chemistry 1999 Jun 18;274(25):17518-24
Membrane topology of NHE3. Epitopes within the carboxyl-terminal hydrophilic domain are exoplasmic.
Biemesderfer D, DeGray B, Aronson PS
The Journal of biological chemistry 1998 May 15;273(20):12391-6
The Journal of biological chemistry 1998 May 15;273(20):12391-6
Monoclonal antibodies for high-resolution localization of NHE3 in adult and neonatal rat kidney.
Biemesderfer D, Rutherford PA, Nagy T, Pizzonia JH, Abu-Alfa AK, Aronson PS
The American journal of physiology 1997 Aug;273(2 Pt 2):F289-99
The American journal of physiology 1997 Aug;273(2 Pt 2):F289-99
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NHE3 Antibody (19F5) [NB110-61586] - Detection of NHE3 in rat kidney lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody (19F5) [NB110-61586] - Analysis of FFPE rat kidney using a 1:25 dilution of NHE3 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Staining was observed in the convoluted tubules and was performed by Histowiz.
