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- Antibody Data
- Antigen structure
- References [13]
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- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB110-81529 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-81529, RRID:AB_1144573
- Product name
- Mouse Monoclonal NHE3/SLC9A3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This antibody detects NHE3 posphorylated at Serine 552, and does not cross-react with the non-phosphopeptide.
- Reactivity
- Human, Mouse, Rat, Porcine, Rabbit, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 2 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Podocyte Injury Augments Intrarenal Angiotensin II Generation and Sodium Retention in a Megalin-Dependent Manner.
Fructose increases the activity of sodium hydrogen exchanger in renal proximal tubules that is dependent on ketohexokinase.
Unmasking a sustained negative effect of SGLT2 inhibition on body fluid volume in the rat.
Resveratrol Inhibits Porcine Intestinal Glucose and Alanine Transport: Potential Roles of Na⁺/K⁺-ATPase Activity, Protein Kinase A, AMP-Activated Protein Kinase and the Association of Selected Nutrient Transport Proteins with Detergent Resistant Membranes.
Renal tubular NHE3 is required in the maintenance of water and sodium chloride homeostasis.
Phospho-NHE3 forms membrane patches and interacts with beta-actin to sense and maintain constant direction during cell migration.
NHE3 phosphorylation via PKCη marks the polarity and orientation of directionally migrating cells.
Gastrin induces sodium-hydrogen exchanger 3 phosphorylation and mTOR activation via a phosphoinositide 3-kinase-/protein kinase C-dependent but AKT-independent pathway in renal proximal tubule cells derived from a normotensive male human.
Natriuretic effect by exendin-4, but not the DPP-4 inhibitor alogliptin, is mediated via the GLP-1 receptor and preserved in obese type 2 diabetic mice.
Persistent directional cell migration requires ion transport proteins as direction sensors and membrane potential differences in order to maintain directedness.
NHE3 function and phosphorylation are regulated by a calyculin A-sensitive phosphatase.
NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity.
Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites.
Koizumi M, Ueda K, Niimura F, Nishiyama A, Yanagita M, Saito A, Pastan I, Fujita T, Fukagawa M, Matsusaka T
Hypertension (Dallas, Tex. : 1979) 2019 Sep;74(3):509-517
Hypertension (Dallas, Tex. : 1979) 2019 Sep;74(3):509-517
Fructose increases the activity of sodium hydrogen exchanger in renal proximal tubules that is dependent on ketohexokinase.
Hayasaki T, Ishimoto T, Doke T, Hirayama A, Soga T, Furuhashi K, Kato N, Kosugi T, Tsuboi N, Lanaspa MA, Johnson RJ, Maruyama S, Kadomatsu K
The Journal of nutritional biochemistry 2019 Sep;71:54-62
The Journal of nutritional biochemistry 2019 Sep;71:54-62
Unmasking a sustained negative effect of SGLT2 inhibition on body fluid volume in the rat.
Masuda T, Watanabe Y, Fukuda K, Watanabe M, Onishi A, Ohara K, Imai T, Koepsell H, Muto S, Vallon V, Nagata D
American journal of physiology. Renal physiology 2018 Sep 1;315(3):F653-F664
American journal of physiology. Renal physiology 2018 Sep 1;315(3):F653-F664
Resveratrol Inhibits Porcine Intestinal Glucose and Alanine Transport: Potential Roles of Na⁺/K⁺-ATPase Activity, Protein Kinase A, AMP-Activated Protein Kinase and the Association of Selected Nutrient Transport Proteins with Detergent Resistant Membranes.
Klinger S, Breves G
Nutrients 2018 Mar 3;10(3)
Nutrients 2018 Mar 3;10(3)
Renal tubular NHE3 is required in the maintenance of water and sodium chloride homeostasis.
Fenton RA, Poulsen SB, de la Mora Chavez S, Soleimani M, Dominguez Rieg JA, Rieg T
Kidney international 2017 Aug;92(2):397-414
Kidney international 2017 Aug;92(2):397-414
Phospho-NHE3 forms membrane patches and interacts with beta-actin to sense and maintain constant direction during cell migration.
Perike S, Özkucur N, Sharma P, Staroske W, Bläsche R, Barth K, Funk RH
Experimental cell research 2014 May 15;324(1):13-29
Experimental cell research 2014 May 15;324(1):13-29
NHE3 phosphorylation via PKCη marks the polarity and orientation of directionally migrating cells.
Özkucur N, Song B, Bola S, Zhang L, Reid B, Fu G, Funk RHW, Zhao M
Cellular and molecular life sciences : CMLS 2014 Dec;71(23):4653-4663
Cellular and molecular life sciences : CMLS 2014 Dec;71(23):4653-4663
Gastrin induces sodium-hydrogen exchanger 3 phosphorylation and mTOR activation via a phosphoinositide 3-kinase-/protein kinase C-dependent but AKT-independent pathway in renal proximal tubule cells derived from a normotensive male human.
Liu T, Jose PA
Endocrinology 2013 Feb;154(2):865-75
Endocrinology 2013 Feb;154(2):865-75
Natriuretic effect by exendin-4, but not the DPP-4 inhibitor alogliptin, is mediated via the GLP-1 receptor and preserved in obese type 2 diabetic mice.
Rieg T, Gerasimova M, Murray F, Masuda T, Tang T, Rose M, Drucker DJ, Vallon V
American journal of physiology. Renal physiology 2012 Oct;303(7):F963-71
American journal of physiology. Renal physiology 2012 Oct;303(7):F963-71
Persistent directional cell migration requires ion transport proteins as direction sensors and membrane potential differences in order to maintain directedness.
Ozkucur N, Perike S, Sharma P, Funk RH
BMC cell biology 2011 Jan 22;12:4
BMC cell biology 2011 Jan 22;12:4
NHE3 function and phosphorylation are regulated by a calyculin A-sensitive phosphatase.
Dynia DW, Steinmetz AG, Kocinsky HS
American journal of physiology. Renal physiology 2010 Mar;298(3):F745-53
American journal of physiology. Renal physiology 2010 Mar;298(3):F745-53
NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity.
Kocinsky HS, Dynia DW, Wang T, Aronson PS
American journal of physiology. Renal physiology 2007 Jul;293(1):F212-8
American journal of physiology. Renal physiology 2007 Jul;293(1):F212-8
Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites.
Kocinsky HS, Girardi AC, Biemesderfer D, Nguyen T, Mentone S, Orlowski J, Aronson PS
American journal of physiology. Renal physiology 2005 Aug;289(2):F249-58
American journal of physiology. Renal physiology 2005 Aug;289(2):F249-58
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Simple Western lane view shows a specific band for NHE3 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Detection of NHE3 [p Ser552] in human kidney lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Mouse intestine, image courtesy of verified customer review.
