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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-51161 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VPS37A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The antibody detects endogenous levels of total VPS37A protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Cellular abundance of sodium phosphate cotransporter SLC20A1/PiT1 and phosphate uptake are controlled post-transcriptionally by ESCRT.
Zechner C, Henne WM, Sathe AA, Xing C, Hernandez G, Sun S, Cheong MC
The Journal of biological chemistry 2022 Jun;298(6):101945
The Journal of biological chemistry 2022 Jun;298(6):101945
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VPS37A was performed by loading Lane 1-2: Human fetal liver tissue and hela cell lysates (40µg) on to a 8% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a VPS37A antibody (Product # PA5-51161) at a 1/200 dilution for 15 seconds, followed by Goat anti rabbit IgG at 1/8000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of VPS37A in paraffin-embedded Human esophagus cancer tissue. Samples were probed using a VPS37A polyclonal antibody (Product # PA5-51161) at a dilution of 1/20.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of VPS37A in paraffin-embedded Human esophagus cancer tissue. Samples were probed using a VPS37A polyclonal antibody (Product # PA5-51161) at a dilution of 1/20.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of VPS37A in paraffin embedded Human Liver cancer tissue using VPS37A Polyclonal Antibody (Product # PA5-51161) at a 1:100 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Genetic and chemical inhibition of ESCRT/lysosomal protein degradation pathway confirm its role as direct negative regulator of SLC20A1 protein levels. A , left , immunoblot of SLC20A1 protein levels in sgVPS37A-HEK293T and sgCHMP6-HEK293T cells with sgControl ( top panel ). Immunoblots of VPS37A ( middle panel ) and CHMP6 ( bottom panel ) were performed for assessment of gene targeting efficiency. beta-actin was used as loading control. Representative data of two experiments are shown. SLC20A1/beta-actin abundance was determined by densitometry and normalized to the control group ( top ). Right , SLC20A1 mRNA expression of sgVPS37A and sgCHMP6 cells with sgControl cells was normalized to 36B4 . n = 4. Bars represent mean +- SD. B , SLC20A1 immunoblot in HEK293T cells treated with lysosome inhibitor bafilomycin A1 (BafA1) or proteasome inhibitor MG-132 versus DMSO control for 24 h ( top panel ). beta-actin was used as loading control ( middle panel ). SLC20A1/beta-actin abundance was determined by densitometry and normalized to the DMSO group ( top ). Efficiency of BafA1 and MG-132 treatment was assessed using ubiquitin immunoblot, which was exposed for optimized visualization of smaller proteins ( bottom panel ). C , HEK293T cells were transfected with CHMP6-EGFP expression plasmid for 24 h ( green in overlayed image) to induce arrest of ESCRT-mediated protein trafficking. Cells were then stained with SLC20A1 antibody and Alexa 594-coupled secondary antibody ( magenta in ove
