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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- 66327-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VPS37A Monoclonal Antibody (1F9A4)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: MPDVPDAFP ELSELSVSQL TDMNEQEEVL LEQFLTLPQL KQIITDKDDL VKSIEELARK NLLLEPSLEA KRQTVLDKYE LLTQMKSTFE KKMQRQHELS ESCSASALQA RLKVAAHEAE EESDNIAEDF LEGKMEIDDF LSSFMEKRTI CHCRRAKEEK LQQAIAMHSQ FHAPL (1-174 aa encoded by BC022363)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1F9A4
- Vial size
- 150 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SW 1990 cells were subjected to SDS PAGE followed by western blot with 66327-1-IG ( VPS37A Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 66327-1-Ig (VPS37A Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol) fixed MCF-7 cells using 66327-1-IG (VPS37A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 66327-1-IG ( VPS37A Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 66327-1-IG ( VPS37A Antibody) at dilution of 1:200 (under 40x lens).
