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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- MAB9030 - Provider product page
- Provider
- R&D Systems
- Product name
- Human KCC4/SLC12A7 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human SLC12A7 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q9Y666- Isotype
- IgG
- Antibody clone number
- 891526
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of KCC4/SLC12A7 in HEK293 Human Cell Line Transfected with Human SLC12A7 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human SLC12A7 or (B) human MRGPRF and eGFP was stained with Mouse Anti-Human KCC4/SLC12A7 Monoclonal Antibody (Catalog # MAB9030) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.
