73-363

antibody from UC Davis/NIH NeuroMab Facility

Targeting: GABRB3

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

73-363

Provider

UC Davis/NIH NeuroMab Facility

Proper citation

UC Davis/NIH NeuroMab Facility Cat#73-363, RRID:AB_2315837

Product name

anti-GABA(A)R, Beta2/3

Antibody type

Monoclonal

Antigen

Affinity purified brain membrane

Reactivity

Human, Mouse, Rat, Bovine, Chicken/Avian

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Fraction of bovine brain GABA-A rec
eptor affinity purified against imm
obilized benzodiazepine Ro7-1986/1
(1988 de Blas et al J Neurosci PMID
2828565, 1988 Vitorica et al J Neu
rosci PMID 2828566). Target initial
ly identified as a GABA-A receptor
by: (1) rat brain immunocytochemist
ry staining pattern via light micro
scopy identical to that seen via li
gand autoradiography with 3H-muscim
ol, (2) immunoprecipitation of 3H-m
uscimol-binding activity of the pur
ified receptor complex and (3) reco
gnition of ~57 kDa protein in immun
oblots against crude rat brain memb
rane fractions. Binding epitope id
entified to be within amino acids 2
6-40 (QSVNDPGNMSFVKET, extracellula
r N-terminus, 1992 Ewert et al Brai
n Res, PMID 1377081) of human GABA-
A-R-Beta3 (also known as Gamma-amin
obutyric acid receptor subunit beta
-3. Binding epitope identified to
be within amino acids 26-40 (QSVNDP
GNMSFVKET, extracellular N-terminus
, 1992 Ewert et al Brain Res, PMID
1377081) of human GABA-A-R-Beta3.


Isotype

IgG

Antibody clone number

62-3G1

Vial size

5000 µl

Concentration

TC Supe

Storage

Antibodies contain 10mm azide. Store at 4°C or aliquot and store at -20°C. Avoid repeated free-thaw cycles.