Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NB300-199 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-199, RRID:AB_2232287
- Product name
- Rabbit Polyclonal GABA-A R beta 3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for the ~53k beta 3-subunit of the GABAA receptor in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references The Na+/H+ Exchanger Nhe1 Modulates Network Excitability via GABA Release.
Effects of Traumatic Stress Induced in the Juvenile Period on the Expression of Gamma-Aminobutyric Acid Receptor Type A Subunits in Adult Rat Brain.
Disinhibition of olfactory bulb granule cells accelerates odour discrimination in mice.
GABA exists as a negative regulator of cell proliferation in spermatogonial stem cells. [corrected].
Hispidulin inhibits the release of glutamate in rat cerebrocortical nerve terminals.
Metabotropic glutamate receptor 5 upregulation in children with autism is associated with underexpression of both Fragile X mental retardation protein and GABAA receptor beta 3 in adults with autism.
GABA(A) receptor downregulation in brains of subjects with autism.
Functional role of GABAergic innervation of the cochlea: phenotypic analysis of mice lacking GABA(A) receptor subunits alpha 1, alpha 2, alpha 5, alpha 6, beta 2, beta 3, or delta.
Rat beta 3 subunits expressed in human embryonic kidney 293 cells form high affinity [35S]t-butylbicyclophosphorothionate binding sites modulated by several allosteric ligands of gamma-aminobutyric acid type A receptors.
Bocker HT, Heinrich T, Liebmann L, Hennings JC, Seemann E, Gerth M, Jakovčevski I, Preobraschenski J, Kessels MM, Westermann M, Isbrandt D, Jahn R, Qualmann B, Hübner CA
Cerebral cortex (New York, N.Y. : 1991) 2019 Sep 13;29(10):4263-4276
Cerebral cortex (New York, N.Y. : 1991) 2019 Sep 13;29(10):4263-4276
Effects of Traumatic Stress Induced in the Juvenile Period on the Expression of Gamma-Aminobutyric Acid Receptor Type A Subunits in Adult Rat Brain.
Lu CY, Liu X, Jiang H, Pan F, Ho CS, Ho RC
Neural plasticity 2017;2017:5715816
Neural plasticity 2017;2017:5715816
Disinhibition of olfactory bulb granule cells accelerates odour discrimination in mice.
Nunes D, Kuner T
Nature communications 2015 Nov 23;6:8950
Nature communications 2015 Nov 23;6:8950
GABA exists as a negative regulator of cell proliferation in spermatogonial stem cells. [corrected].
Du Y, Du Z, Zheng H, Wang D, Li S, Yan Y, Li Y
Cellular & molecular biology letters 2013 Jun;18(2):149-62
Cellular & molecular biology letters 2013 Jun;18(2):149-62
Hispidulin inhibits the release of glutamate in rat cerebrocortical nerve terminals.
Lin TY, Lu CW, Wang CC, Lu JF, Wang SJ
Toxicology and applied pharmacology 2012 Sep 1;263(2):233-43
Toxicology and applied pharmacology 2012 Sep 1;263(2):233-43
Metabotropic glutamate receptor 5 upregulation in children with autism is associated with underexpression of both Fragile X mental retardation protein and GABAA receptor beta 3 in adults with autism.
Fatemi SH, Folsom TD, Kneeland RE, Liesch SB
Anatomical record (Hoboken, N.J. : 2007) 2011 Oct;294(10):1635-45
Anatomical record (Hoboken, N.J. : 2007) 2011 Oct;294(10):1635-45
GABA(A) receptor downregulation in brains of subjects with autism.
Fatemi SH, Reutiman TJ, Folsom TD, Thuras PD
Journal of autism and developmental disorders 2009 Feb;39(2):223-30
Journal of autism and developmental disorders 2009 Feb;39(2):223-30
Functional role of GABAergic innervation of the cochlea: phenotypic analysis of mice lacking GABA(A) receptor subunits alpha 1, alpha 2, alpha 5, alpha 6, beta 2, beta 3, or delta.
Maison SF, Rosahl TW, Homanics GE, Liberman MC
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Oct 4;26(40):10315-26
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Oct 4;26(40):10315-26
Rat beta 3 subunits expressed in human embryonic kidney 293 cells form high affinity [35S]t-butylbicyclophosphorothionate binding sites modulated by several allosteric ligands of gamma-aminobutyric acid type A receptors.
Slany A, Zezula J, Tretter V, Sieghart W
Molecular pharmacology 1995 Sep;48(3):385-91
Molecular pharmacology 1995 Sep;48(3):385-91
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GABA-A R beta 3 Antibody [NB300-199] - Rat brain lysate showing specific immunolabeling of the 53 kDa Beta(3)-subunit of the GABA(A)-R
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GABA-A R beta 3 Antibody [NB300-199] - Western blot of whole-olfactory bulb homogenates using the B3-subunit antibody in rAAV-Cre-injected versus non-injected GABRB3loxP/loxP mice (each six samples from three mice). The B3-subunit appears as a band of 55 kDa, consistent with its predicted molecular weight. Densitometric analysis (mean+/-s.e.m.) revealed a significant difference (t-test; *P=0.016). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/ncomms9950) licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199] - Immunostaining of mouse retina showing specific labeling of the GABAA beta3 subunit in green, calbindin in red and DNA in blue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GABA-A R beta 3 Antibody [NB300-199] - Paraffin-embedded, formalin-fixed mouse head section. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199] - Each channel was filtered frame by frame. The raw data was acquired at high confocal resolution (XY: 234.32 um x 234.32 um, pixel size = 0.114 um) using a 63x glycerol objective (NA=1.3). Z-steps were 0.15 um per frame. Acquired raw data (1) was filtered using an anisotropic diffusion 2D filter (2). (5). The final images (6) were obtained by thresholding the immunosignal. Scale bars: 10um. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/ncomms9950) licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: GABA-A R beta 3 Antibody [NB300-199] - Clustered distribution of the GABA-A R beta 3-subunit. Immunostaining using aG ABA-A R beta 3-subunit-selective antibody (red hot colour palette) in horizontal OB slices infected with rAAV-mGFP (grey). The images represent single confocal frames. From left to right, GCL, MC layer (MCL), EPL and glomerular layer (GL). Scale bar, 200 um. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/ncomms9950) licensed under a CC-BY licence.