Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [5]
- Other assay [1]
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Validation data
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- Product number
- 66625-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RIC8A Monoclonal Antibody (1E3B11)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: QQLFQELKG VRLLTDTLEL TLGVTPEGNP PTLLPSQETE RAMEILKVLF NITLDSIKGE VDEEDAALYR HLGTLLRHCV MIATAGDRTE EFHGHAVNLL GNLPLKCLDV LLTLEPHGDS TEFMGVNMDV IRALLIFLEK RLHKTHRLKE SVAPVLSVLT ECARMHRPAR KFLKAQVLPP LRDVRTRPEV GEMLRNKLVR LMTHLDTDVK RVAAEFLFVL CSESVPRFIK YTGYGNAAGL LAARGLMAGG RPEGQYSEDE DTDTDEYKEA KASINPVTGR VEEKPPNPME GMTEEQKEHE AMKLVTMFDK LSRNRVIQPM GMSPRGHLTS LQDAMCETME QQLSSDPDSD PD (117-467 aa encoded by BC011821)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1E3B11
- Vial size
- 150 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 66625-1-IG (RIC8A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 66625-1-Ig (RIC8A antibody) at dilution of 1:500 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 66625-1-Ig (RIC8A antibody) at dilution of 1:500 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 66625-1-IG (RIC8A antibody) at dilution of 1:500 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 66625-1-IG (RIC8A antibody) at dilution of 1:500 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RIC8A antibody (Product # 66625-1-IG, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-RIC8A (IP:66625-1-IG, 5ug; Detection:66625-1-IG 1:2000) with HEK-293 cells lysate 4000 ug.