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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- GTX83618 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX83618, RRID:AB_10728197
- Product name
- SLC7A8 antibody [5A9]
- Antibody type
- Monoclonal
- Reactivity
- Human, Simian
- Host
- Mouse
Submitted references LAT2 regulates glutamine-dependent mTOR activation to promote glycolysis and chemoresistance in pancreatic cancer.
Feng M, Xiong G, Cao Z, Yang G, Zheng S, Qiu J, You L, Zheng L, Zhang T, Zhao Y
Journal of experimental & clinical cancer research : CR 2018 Nov 12;37(1):274
Journal of experimental & clinical cancer research : CR 2018 Nov 12;37(1):274
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC7A8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC7A8.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-SLC7A8 mouse monoclonal antibody (GTX83618) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SLC7A8
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of SLC7A8 by using TrueMab monoclonal anti-SLC7A8 antibodies (Negative control: IP without adding anti-SLC7A8 antibody.). For each experiment, 500ul of DDK tagged SLC7A8 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SLC7A8 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-SLC7A8 mouse monoclonal antibody. (GTX83618)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY SLC7A8
