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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX83621 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX83621, RRID:AB_10728196
- Product name
- SLC7A8 antibody [4H10]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-SLC7A8 mouse monoclonal antibody (GTX83621) immunofluorescent staining of COS7 cells transiently transfected with SLC7A8
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using anti-SLC7A8 mouse monoclonal antibody (GTX83621).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of SLC7A8 by using TrueMab monoclonal anti-SLC7A8 antibodies (Negative control: IP without adding anti-SLC7A8 antibody.). For each experiment, 500ul of DDK tagged SLC7A8 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SLC7A8 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-SLC7A8 antibody(GTX83621),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
