FNab08156

antibody from FineTest Biotech

Targeting: SPAST ADPSP, FSP2, KIAA1083, SPG4

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Antibody Data

Product number

FNab08156

Provider

FineTest Biotech

Product name

SPAST antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated(PubMed:19141076 PubMed:20530212). Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold(By similarity). Severing activity is not dependent on tubulin acetylation or detyrosination(By similarity). Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation(By similarity). It is critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia(By similarity). SPAST is involved in abscission step of cytokinesis and nuclear envelope reassembly during anaphase in cooperation with the ESCRT-III complex(By similarity). Recruited at the midbody, probably by IST1, and participates to membrane fission during abscission together with the ESCRT-III complex(By similarity). Recruited to the nuclear membrane by IST1 and mediates microtubule severing, promoting nuclear envelope sealing and mitotic spindle disassembly during late anaphase(By similarity). Required for membrane traffic from the endoplasmic reticulum(ER) to the Golgi and endosome recycling(By similarity). Recruited by IST1 to endosomes and regulates early endosomal tubulation and recycling by mediating microtubule severing(By similarity). Probably plays a role in axon growth and the formation of axonal branches(PubMed:18234839). Isoform 1: Involved in lipid metabolism by regulating the size and distribution of lipid droplets.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

spastin


Epitope

IgG

Isotype

IgG

Vial size

100¦Ìg

Concentration

Immunogen affinity purified

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20¡æ for 12 months(Avoid repeated freeze / thaw cycles.)

Handling

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20¡æ for 12 months(Avoid repeated freeze / thaw cycles.)