NBP1-81586

antibody from Novus Biologicals

Targeting: PLA2G6 iPLA2, iPLA2beta, NBIA2, PARK14, PNPLA9

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-81586

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-81586, RRID:AB_11016238

Product name

Rabbit Polyclonal PLA2G6 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human PLA2G6 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Oligomeric amyloid-beta induces MAPK-mediated activation of brain cytosolic and calcium-independent phospholipase A(2) in a spatial-specific manner.

Palavicini JP, Wang C, Chen L, Hosang K, Wang J, Tomiyama T, Mori H, Han X
Acta neuropathologica communications 2017 Jul 27;5(1):56

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PLA2G6 accumulates in Lewy bodies in PARK14 and idiopathic Parkinson's disease.

Miki Y, Tanji K, Mori F, Kakita A, Takahashi H, Wakabayashi K
Neuroscience letters 2017 Apr 3;645:40-45

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Group VIA phospholipase A2 is a target for vasopressin signaling in the thick ascending limb.

Paliege A, Roeschel T, Neymeyer H, Seidel S, Kahl T, Daigeler AL, Mutig K, Mrowka R, Ferreri NR, Wilson BS, Himmerkus N, Bleich M, Bachmann S
American journal of physiology. Renal physiology 2012 Apr 1;302(7):F865-74

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PLA2G6 Antibody [NBP1-81586] - Analysis in human cell line HEL.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PLA2G6 Antibody [NBP1-81586] - Effects of APPWT and APPOSK overexpression on the levels of the major phospholipase A2s in the brains of old mice. Cerebrum samples from non-Tg, APPWT and APPOSK were lyophilized, pulverized, and homogenized in NP40 buffer using a cooled bead beater. Total protein concentrations from NP40 supernatants were estimated by BCA protein assay. Representative Western blots using multiple antibodies against PLA2. Image collected and cropped by CiteAb from the following publication (http://actaneurocomms.biomedcentral.com/articles/10.1186/s40478-017-0460-6), licensed under a CC-BY licence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PLA2G6 Antibody [NBP1-81586] - Analysis in control (vector only transfected HEK293T lysate) and PLA2G6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: PLA2G6 Antibody [NBP1-81586] - Non-Tg mouse brains were dissected, fixed in 4% paraformaldehyde, cryoprotected, frozen, & sectioned coronally (8 um sections). Representative immunofluorescence images taken from brain coronal sections (Bregma -2) using phospho-cPLA2 (yellow) antibody and DAPI (blue) (a) or and iPLA2 (yellow) and NeuN (red) antibodies (b). Images show a whole hemibrain section using a 20x objective on a Nikon A1R VAAS inverted confocal (left panels). NeuN and iPLA2 co-localize within the perinucleus of pyramidal neurons (PN, white arrows), but not within the perinucleus of interneurons (IN). iPLA2 staining is also observed within pyramidal dendritic arborization (right panels) Image collected and cropped by CiteAb from the following publication (http://actaneurocomms.biomedcentral.com/articles/10.1186/s40478-017-0460-6), licensed under a CC-BY licence.