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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- NBP1-42677 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-42677, RRID:AB_2235620
- Product name
- Rabbit Polyclonal MTMR15 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MTMR15 Antibody [NBP1-42677] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MTMR15 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. MTMR15 was also immunoprecipitated by another rabbit anti-MTMR15 antibody, which recognizes a downstream epitope.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MTMR15 Antibody [NBP1-42677] - Detection of human MTMR15 by western blot. Samples: Whole cell lysate (50 ug) from GaMG, HeLa, Jurkat, and My-La CD4+ cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MTMR15 antibody NBP1-42677 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MTMR15 Antibody [NBP1-42677] - FFPE section of human ovarian carcinoma. Dilution of 1:1,000 (1ug/ml).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MTMR15 Antibody [NBP1-42677] - FFPE section of human ovarian carcinoma. Antibody used at a dilution of 1:250 Detection: DAB staining using Immunohistochemistry Accessory Kit.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MTMR15 Antibody [NBP1-42677] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MTMR15 (NBP1-42677) used at a dilution of 1:200 (1ug/ml). Detection: DAB
