NBP1-28737

antibody from Novus Biologicals

Targeting: POLR3D BN51T, C53, RPC4, TSBN51

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-28737

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-28737, RRID:AB_1913975

Product name

Rabbit Polyclonal POLR3D Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: POLR3D Antibody [NBP1-28737] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-POLR3D antibody NBP1-28737 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. POLR3D was also immunoprecipitated by rabbit anti-POLR3D antibody NBP1-28726, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: POLR3D Antibody [NBP1-28737] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- POLR3D used at a dilution of 1:1,000 (0.2 ug/ml). Detection: DAB