Explore
Validate
Learn12093-1-AP
antibody from Invitrogen Antibodies
Targeting: UBA5
FLJ23251, UBE1DC1
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 12093-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UBA5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: YDKVELANM NRLFFQPHQA GLSKVQAAEH TLRNINPDVL FEVHNYNITT VENFQHFMDR ISNGGLEEGK PVDLVLSCVD NFEARMTINT ACNELGQTWM ESGVSENAVS GHIQLIIPGE SACFACAPPL VVAANIDEKT LKREGVCAAS LPTTMGVVAG ILVQNVLKFL LNFGTVSFYL GYNAMQDFFP TMSMKPNPQC DDRNCRKQQE EYKKKVAALP KQEVIQEEEE IIHEDNEWGI ELVSEVSEEE LKNFSGPVPD LPEGITVAYT IPKKQEDSVT ELTVEDSGES LEDLMAKMKN M (105-404 aa encoded by BC009737)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 12093-1-AP (UBA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 12093-1-AP ( UBA5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12093-1-AP (UBA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human colon tissue were subjected to SDS PAGE followed by western blot with 12093-1-AP (UBA5 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using UBA5 antibody 12093-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 12093-1-AP (UBA5 antibody) at dilution of 1:100 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug UBA5 antibody (12093-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-UBA5 (IP:12093-1-AP, 4ug; Detection:12093-1-AP 1:1000) with HeLa cells lysate 1800ug.
