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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-93730 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-93730, RRID:AB_11040564
- Product name
- Rabbit Polyclonal GRAMD1A Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human GRAMD1A antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The cholesterol transfer protein GRAMD1A regulates autophagosome biogenesis.
Laraia L, Friese A, Corkery DP, Konstantinidis G, Erwin N, Hofer W, Karatas H, Klewer L, Brockmeyer A, Metz M, Schölermann B, Dwivedi M, Li L, Rios-Munoz P, Köhn M, Winter R, Vetter IR, Ziegler S, Janning P, Wu YW, Waldmann H
Nature chemical biology 2019 Jul;15(7):710-720
Nature chemical biology 2019 Jul;15(7):710-720
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GRAMD1A Antibody [NBP1-93730] - Analysis in human cell lines A-549 and MCF-7 using anti-GRAMD1A antibody. Corresponding GRAMD1A RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRAMD1A Antibody [NBP1-93730] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
