- PA5-27614 - Invitrogen Antibodies TALDO1 antibody

Dewdney B, Alanazy M, Gillman R, Walker S, Wankell M, Qiao L, George J, Roberts A, Hebbard L
Scientific reports 2020 Oct 7;10(1):16769

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of HL-60 (Lane 1), MOLT-4 (Lane 2), U-937 (Lane 3), K-562 (Lane 4), Hep G2 (Lane 5), U-87 MG (Lane 6), tissue extracts of Mouse Brain (Lane 7), Rat Liver (Lane 8) and Human PBMC (Lane 9). The blot was probed with Anti-TALDO1 Polyclonal Antibody (Product # PA5-27614, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 38 kDa band corresponding to TALDO1 was observed across the cell lines and tissues tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using TALDO1 Polyclonal Antibody (Product # PA5-27614). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. TALDO1 Polyclonal Antibody (Product # PA5-27614) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using TALDO1 Polyclonal Antibody (Product # PA5-27614). Sample (30 µg of whole cell lysate). Lane A: A431. 10% SDS PAGE. TALDO1 Polyclonal Antibody (Product # PA5-27614) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Knockdown of TALDO1 was achieved by transfecting Hep G2 cells with TALDO1 specific siRNAs (Silencer® select Product # s13775, s13777). Western blot analysis (Fig. a) was performed using whole cell extracts from the TALDO1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with TALDO1 Polyclonal Antibody (Product # PA5-27614, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to TALDO1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: TALDO1 Polyclonal Antibody detects TALDO1 protein at nucleus on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. TALDO1 Polyclonal Antibody (Product # PA5-27614) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 1 Evaluating A52 and Huh7 HCC growth and protein expression in glucose and fructose conditions. Cell proliferation via a BrdU assay was determined for ( a ) A52 cells, and ( b ) Huh7 cells grown in media containing 5 mM glucose or 5 mM fructose over 24, 48 and 72 h. Growth of ( c ) A52 and ( d ) Huh7 tumours was evaluated in mice fed a normal or high-fructose chow. Western blots of cell lysates for TKT, TALDO, PHGDH, G6PD, PSAT1 and beta-actin from, ( e ) A52 cells, and ( f ) Huh7 cells growth in glucose or fructose containing media for 24 and 48 h, and tumour lysates from ( g ) A52, and ( h ) Huh7 (H) subcutaneous tumours in mice fed a normal chow or high fructose diet. Original blots and densitometry analysis for the in vitro protein analysis and in vivo protein analysis are shown in Supplementary Figures 1 - 8 (Supplementary File 1 ). Full length western blot images for TKT, TALDO, PHGDH, PSAT1 and beta-actin could not be provided for ( e ) and ( g ). These images were provided from the thesis of M.A., and full-length original images could not be obtained. The original images in this figure and the Supplementary File are as presented in the thesis of M.A. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. G6PD glucose-6-phosphate dehydrogenase, PHGDH phosphoglycerate dehydrogenase, PSAT1 phosphoserine aminotransferase1, TALDO transaldolase, TKT transketolase.

PA5-27614