H00005522-M01

antibody from Abnova Corporation

Targeting: PPP2R2C B55gamma, IMYPNO, MGC33570, PR52, PR55G

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005522-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005522-M01, RRID:AB_606826

Product name

PPP2R2C monoclonal antibody (M01), clone 6D1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant PPP2R2C.

Antigen sequence

MGEDTDTRKINHSFLRDHSYVTEADIISTVEFNHT
GELLATGDKGGRVVIFQREPESKNAPHSQGEYDVY
STFQSHEPEFDYLKSLEIEEKINKIKWLPQQNAAH
SLLST


Isotype

IgG

Antibody clone number

6D1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

PPP2R2C loss promotes castration-resistance and is associated with increased prostate cancer-specific mortality.

Bluemn EG, Spencer ES, Mecham B, Gordon RR, Coleman I, Lewinshtein D, Mostaghel E, Zhang X, Annis J, Grandori C, Porter C, Nelson PS
Molecular cancer research : MCR 2013 Jun;11(6):568-78

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

PPP2R2C monoclonal antibody (M01), clone 6D1 Western Blot analysis of PPP2R2C expression in HeLa ( Cat # L013V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PPP2R2C expression in transfected 293T cell line by PPP2R2C monoclonal antibody (M01), clone 6D1.Lane 1: PPP2R2C transfected lysate(49.5 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PPP2R2C is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol