H00008079-M01

antibody from Abnova Corporation

Targeting: MLF2 NTN4

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00008079-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00008079-M01, RRID:AB_425757

Product name

MLF2 monoclonal antibody (M01), clone 2F6-1E3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant MLF2.

Antigen sequence

MFRFMRDVEPEDPMFLMDPFAIHRQHMSRMLSGGF
GYSPFLSITDGNMPGTRPASRRMQQAGAVSPFGML
GMSGGFMDMFGMMNDMIGNMEHMTAGGNCQTFSSS
TVISYSNTGDGAPKVYQETSEMRSAPGGIRETRRT
VRDSDSGLEQMSIGHHIRDRAHILQRSRNHRTGDQ
EERQDYINLDESEAAAFDDEWRRETSRFRQQRPLE
FRRLESSGAGGRRAEGPPRLAIQGPEDSPSRQSRR
YDW


Isotype

IgG

Antibody clone number

2F6-1E3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MLF2 expression in transfected 293T cell line by MLF2 monoclonal antibody (M01), clone 2F6-1E3.Lane 1: MLF2 transfected lysate(27.28 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged MLF2 is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to MLF2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol