Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [8]
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Validation data
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- Product number
- LS-C782216 - Provider product page
- Provider
- LSBio
- Product name
- GOLPH3 Antibody LS-C782216
- Antibody type
- Polyclonal
- Description
- Antigen Affinity purification
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Storage
- After reconstitution, store at 4°C for up to 1 month. Long-term: aliquot and store at -20°C. Avoid freeze-thaws cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) placenta, 3) U-2 OS, 4) Caco-2, 5) SW620 and 6) A549 lysate with GOLPH3 antibody at 0.5ug/ml. Predicted molecular weight ~34 kDa.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot testing of rat 1) heart, 2) brain, 3) stomach, 4) testis and mouse 5) heart, 6) brain, 7) stomach, 8) testis and 9) NIH3T3 lysate with GOLPH3 antibody at 0.5ug/ml. Predicted molecular weight ~34 kDa.
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC staining of FFPE rat intestine with GOLPH3 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC staining of FFPE human lung cancer with GOLPH3 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC staining of FFPE human intestinal cancer with GOLPH3 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC staining of FFPE rat brain with GOLPH3 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC staining of FFPE human lung cancer with GOLPH3 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC staining of FFPE human intestinal cancer with GOLPH3 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat brain with GOLPH3 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat intestine with GOLPH3 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.